Lennart Hilbert, to be
Junior Professor for Systems Biology/Bioinformatics,
starting October 1st, 2018

Karlsruhe Institute of Technology (KIT),
Institute of Toxicology and Genetics (ITG)
and Zoological Institute, Faculty of Chemistry and Biosciences

Research Program: Computational Architectures in the Cell Nucleus

A hand-drawn sketch displaying the background and main concepts of the research program of the Hilbert lab.
“On-a-napkin” version of our research program

The increasing need for data storage and ambitions for bio-compatible computing mark DNA computation as a critical future technology. DNA-based hardware, however, will be rooted in physical principles that fundamentally differ from electronic hardware. For example, information processing in dense suspensions of DNA will be severely limited by slow transport of information and occurs in a liquid, rather than in a static architecture. Also, like electronic computer chips in cellular devices, DNA hardware will only achieve its full potential once it can be ubiquitously embedded in biological cells and biotechnological devices.

Luckily, these challenges have already been overcome by the nucleus of the biological cell. The cell nucleus stores and manages access to more than 1 GB of DNA-encoded information. This genetic information is accessed in a massively parallel fashion, and processes that are based on this genetic information are fully integrated with cellular functions. Recent work, including our own, revealed that the targeted read-out of genetic information is closely connected to the three-dimensional (3D) organization of DNA in the cell nucleus: regions of the genome that are expressed become unfolded, regions that are repressed become compacted. Assuming that this adaptive 3D organization of DNA is central to effective information processing in the cell nucleus, we will

  • explore the physical principles driving this adaptive 3D organization, using major gene regulatory transitions as biomimetic examples,
  • investigate how this adaptive 3D organization can gate the access of regulatory factors to the genome, or, in other words, how 3D organization can gate the information flow into and out of different regions of the genome,
  • exploit the above physical principles in the development of cell-embedded DNA computing modules that are switched on/off by 3D unfolding/compaction, and, potentially, the in vitro synthesis of DNA-based hardware.

Our techniques include molecular and cellular work with vertebrate cells (especially zebrafish), live and super-resolution microscopy, advanced image analysis, as well as computer simulations and (bio)physical modeling. Beyond our core team, we benefit from a strong environment of interdisciplinary collaborators as well as extensive animal, imaging, and computing infrastructures.

Last but not least, we have different opportunities for creative and committed applicants at any career level, with experimental, theoretical, computational and/or any other background you feel might contribute to our vision. Most importantly, we are looking for the readiness to prioritize exciting scientific questions over discipline boundaries. In our search for candidates, we would like to access the whole talent pool – meaning that we especially hope for applications from women and also minorities, disadvantaged individuals, or persons coming out of a not-so-straight career path. Your applications will be given special attention; please indicate such relevant circumstances if not obvious from your application, so they will be considered. Please send formal inquiries and/or full application packages to lennart.hilbert@gmail.com, also feel free to tweet or message, or simply cold-approach us at meetings.

Disclaimer: This research outline and its statements are an interim solution for the transition period to Karlsruhe Institute of Technology. This text is not reviewed or endorsed by Karlsruhe Institute of Technology, hosted on a private website, and as a consequence is not legally binding.